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meta-analysis of RNA-seq data using metaRNASeq package


R reading annotation columns by read.ilmn of package limmadata.table vs dplyr: can one do something well the other can't or does poorly?Complex clustering of RNA-seq dataPerform multiple survival analysis with loop in RRNA-Seq Differential Expression - DESeqDifferential gene expression analysis in PythonHow to filter genes from seuratobject in slotname @data?Rsubread: 'ERROR: cannot finish the SAM file!'Deseq2 multiple group comparisonhisat2-stringtie-ballgown pipeline gives high p-value and q-value for RNA-seq of Arabidopsis thaliana






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I'm trying to run a meta-analysis from different RNA-Seq datasets using the package metaRNASeq. I have run the differential expression analysis for the individual datasets using DESeq2 and I obtained the differential expressed genes and the p-values. DESeq2 gives a two-tailed p-value. My question is: should I transform the p-values from two-tailed to one-tailed or I can use them directly as two-tailed to perform the meta-analysis using metaRNASeq package? Thanks in advance!!










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    0















    I'm trying to run a meta-analysis from different RNA-Seq datasets using the package metaRNASeq. I have run the differential expression analysis for the individual datasets using DESeq2 and I obtained the differential expressed genes and the p-values. DESeq2 gives a two-tailed p-value. My question is: should I transform the p-values from two-tailed to one-tailed or I can use them directly as two-tailed to perform the meta-analysis using metaRNASeq package? Thanks in advance!!










    share|improve this question
























      0












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      0








      I'm trying to run a meta-analysis from different RNA-Seq datasets using the package metaRNASeq. I have run the differential expression analysis for the individual datasets using DESeq2 and I obtained the differential expressed genes and the p-values. DESeq2 gives a two-tailed p-value. My question is: should I transform the p-values from two-tailed to one-tailed or I can use them directly as two-tailed to perform the meta-analysis using metaRNASeq package? Thanks in advance!!










      share|improve this question














      I'm trying to run a meta-analysis from different RNA-Seq datasets using the package metaRNASeq. I have run the differential expression analysis for the individual datasets using DESeq2 and I obtained the differential expressed genes and the p-values. DESeq2 gives a two-tailed p-value. My question is: should I transform the p-values from two-tailed to one-tailed or I can use them directly as two-tailed to perform the meta-analysis using metaRNASeq package? Thanks in advance!!







      r rna-seq






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      asked Mar 22 at 16:44









      MonahAbouAlezzMonahAbouAlezz

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